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cd147 emmprin  (R&D Systems)


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    Structured Review

    R&D Systems cd147 emmprin
    Cd147 Emmprin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+basigin/Mouse+EMMPRIN%2FCD147+Antibody/pmc12848544-302-18-24
    Average 88 stars, based on 4 article reviews
    cd147 emmprin - by Bioz Stars, 2026-09
    88/100 stars

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    Related Articles

    Western Blot:

    Article Title: In Situ trans Ligands of CD22 Identified by Glycan-Protein Photocross-linking-enabled Proteomics
    Article Snippet: Human and murine B-cells were enriched from blood and spleen, respectively, using B-cell negative isolation kits (Dynal, Invitrogen). .. Antibodies and dilutions for Western blots were as follows: anti-CD22 (Santa Cruz Biotechnology Inc., sc-7932, 1:500), anti-CD45 (Santa Cruz Biotechnology Inc., sc-25590, 1:500), anti-4F2 (Santa Cruz Biotechnology Inc., sc-9160, 1:200), anti-major histocompatibility complex I (Santa Cruz Biotechnology Inc., sc-25619, 1:200), anti-Plexin-B2 (Santa Cruz Biotechnology Inc., sc-34504, 1:200), anti-ST6GalI (Santa Cruz Biotechnology Inc., sc-20926, 1:200), anti-HLA II DRα (Dako, M0746, 1:200), anti-Basigin (R&D Systems, MAB972, 1:200), anti-Siglec-10 (R&D Systems, AF2130, 1:200), anti-transferrin receptor (Invitrogen, A-11130, 1:200), anti-IgM-HRP (Bethyl Laboratories, A80–100P, 1:2000). .. HRP-conjugated secondary antibodies were obtained from Jackson ImmunoResearch Laboratories.

    Article Title: In Situ trans Ligands of CD22 Identified by Glycan-Protein Photocross-linking-enabled Proteomics
    Article Snippet: Human and murine B-cells were enriched from blood and spleen, respectively, using B-cell negative isolation kits (Dynal, Invitrogen). .. Antibodies Antibodies and dilutions for Western blots were as follows: anti-CD22 (Santa Cruz Biotechnology Inc., sc-7932, 1:500), anti-CD45 (Santa Cruz Biotechnology Inc., sc-25590, 1:500), anti-4F2 (Santa Cruz Biotechnology Inc., sc-9160, 1:200), anti-major histocompatibility complex I (Santa Cruz Biotechnology Inc., sc-25619, 1:200), anti-Plexin-B2 (Santa Cruz Biotechnology Inc., sc-34504, 1:200), anti-ST6GalI (Santa Cruz Biotechnology Inc., sc-20926, 1:200), anti-HLA II DRα (Dako, M0746, 1:200), anti-Basigin (R&D Systems, MAB972, 1:200), anti-Siglec-10 (R&D Systems, AF2130, 1:200), anti-transferrin receptor (Invitrogen, A-11130, 1:200), anti-IgM-HRP (Bethyl Laboratories, A80–100P, 1:2000). .. HRP-conjugated secondary antibodies were obtained from Jackson ImmunoResearch Laboratories.

    Saline:

    Article Title: Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca 2+ -ATPases and Key Regulators of Ca 2+ Clearance.
    Article Snippet: .. Replicas were washed in 50 mM tris-buffered saline (TBS) containing 0.05% bovine serum albumin (BSA, Roth, Germany) and 0.1% Tween20 (Tw20, Roth) and then incubated in a blocking solution (5% BSA) and then in either one the following primary antibodies: anti-PMCA1 (ACP-005, Alomone, 2.4 mg/ml), anti-PMCA2 (ab3529, Abcam, 3.1 mg/mL), anti-Basigin (AF772, R&D Systems, 3.1 mg/mL) or in a mixture of anti-PMCA2 and anti-vesicular glutamate transporter-1 (v-GluT-1-Go-Af310, Frontier Institue, 0.5 mg/mL) in 50 mM TBS containing 1%BSA and 0.1% Tw20 O/N at room temperature. .. Subsequently, replicas were reacted with 12 nm gold-coupled secondary antibodies or a mixture of 12 nm and 18 nm goldcoupled antibodies (1:30, Jackson ImmunoResearch Laboratories, PA) made up in 50mM TBS containing 5%BSAO/N at 15 C. The labeled replicas were examined using a transmission electron microscope (Zeiss, LEO 906E, Germany).

    Incubation:

    Article Title: Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca 2+ -ATPases and Key Regulators of Ca 2+ Clearance.
    Article Snippet: .. Replicas were washed in 50 mM tris-buffered saline (TBS) containing 0.05% bovine serum albumin (BSA, Roth, Germany) and 0.1% Tween20 (Tw20, Roth) and then incubated in a blocking solution (5% BSA) and then in either one the following primary antibodies: anti-PMCA1 (ACP-005, Alomone, 2.4 mg/ml), anti-PMCA2 (ab3529, Abcam, 3.1 mg/mL), anti-Basigin (AF772, R&D Systems, 3.1 mg/mL) or in a mixture of anti-PMCA2 and anti-vesicular glutamate transporter-1 (v-GluT-1-Go-Af310, Frontier Institue, 0.5 mg/mL) in 50 mM TBS containing 1%BSA and 0.1% Tw20 O/N at room temperature. .. Subsequently, replicas were reacted with 12 nm gold-coupled secondary antibodies or a mixture of 12 nm and 18 nm goldcoupled antibodies (1:30, Jackson ImmunoResearch Laboratories, PA) made up in 50mM TBS containing 5%BSAO/N at 15 C. The labeled replicas were examined using a transmission electron microscope (Zeiss, LEO 906E, Germany).

    Article Title: Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca 2+ -ATPases and Key Regulators of Ca 2+ Clearance.
    Article Snippet: .. Solubilisates (500 or 1000 ml) were incubated for 2 hr at 4 C with the following immobilized antibodies (ABs; each 10 mg): anti-PMCA pan (‘anti-PMCA-a’, MA3-914 (clone 5F10), Thermo Fisher Scientific), anti-PMCA1/4 (‘anti-PMCA-b‘, sc-71907, Santa Cruz Biotechnology), and anti-neuroplastin-a (AF7818), anti-neuroplastin-b (AF5360), anti-basigin (AF772), all from R&D Systems. ..

    Blocking Assay:

    Article Title: Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca 2+ -ATPases and Key Regulators of Ca 2+ Clearance.
    Article Snippet: .. Replicas were washed in 50 mM tris-buffered saline (TBS) containing 0.05% bovine serum albumin (BSA, Roth, Germany) and 0.1% Tween20 (Tw20, Roth) and then incubated in a blocking solution (5% BSA) and then in either one the following primary antibodies: anti-PMCA1 (ACP-005, Alomone, 2.4 mg/ml), anti-PMCA2 (ab3529, Abcam, 3.1 mg/mL), anti-Basigin (AF772, R&D Systems, 3.1 mg/mL) or in a mixture of anti-PMCA2 and anti-vesicular glutamate transporter-1 (v-GluT-1-Go-Af310, Frontier Institue, 0.5 mg/mL) in 50 mM TBS containing 1%BSA and 0.1% Tw20 O/N at room temperature. .. Subsequently, replicas were reacted with 12 nm gold-coupled secondary antibodies or a mixture of 12 nm and 18 nm goldcoupled antibodies (1:30, Jackson ImmunoResearch Laboratories, PA) made up in 50mM TBS containing 5%BSAO/N at 15 C. The labeled replicas were examined using a transmission electron microscope (Zeiss, LEO 906E, Germany).



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    Clinacanthus nutans (Burm.f.) Lindau (CN) attenuates tumorigenicity of colon cancer in vivo. A The total ion chromatography of CN with top 10 chemical components labelled in the positive electrospray ionization modes. B The body weight curves of each group were recorded. C Statistical data of tumor weight in each group. D Statistical data of tumor volume in each group. E Representative pictures of mouse tumors in groups. F H&E staining of tissue sections. Amplification: 400 × , scale bar: 20 μm. G IHC analysis of Ki67 for tissues of xenograft tumors. Amplification: 400 × , scale bar: 20 μm. (H) VEGF, <t>CD147</t> and MMP-9 protein expressions were detected by Western blot. ** p < 0.01, *** p < 0.001 vs. Colon cancer; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. Low-CN
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    Image Search Results


    Clinacanthus nutans (Burm.f.) Lindau (CN) attenuates tumorigenicity of colon cancer in vivo. A The total ion chromatography of CN with top 10 chemical components labelled in the positive electrospray ionization modes. B The body weight curves of each group were recorded. C Statistical data of tumor weight in each group. D Statistical data of tumor volume in each group. E Representative pictures of mouse tumors in groups. F H&E staining of tissue sections. Amplification: 400 × , scale bar: 20 μm. G IHC analysis of Ki67 for tissues of xenograft tumors. Amplification: 400 × , scale bar: 20 μm. (H) VEGF, CD147 and MMP-9 protein expressions were detected by Western blot. ** p < 0.01, *** p < 0.001 vs. Colon cancer; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. Low-CN

    Journal: Discover Oncology

    Article Title: Clinacanthus nutans (Burm.f.) Lindau facilitates cuproptosis and ameliorates colon cancer progression by inhibiting PDE3B-mediated Apelin pathway

    doi: 10.1007/s12672-025-02037-w

    Figure Lengend Snippet: Clinacanthus nutans (Burm.f.) Lindau (CN) attenuates tumorigenicity of colon cancer in vivo. A The total ion chromatography of CN with top 10 chemical components labelled in the positive electrospray ionization modes. B The body weight curves of each group were recorded. C Statistical data of tumor weight in each group. D Statistical data of tumor volume in each group. E Representative pictures of mouse tumors in groups. F H&E staining of tissue sections. Amplification: 400 × , scale bar: 20 μm. G IHC analysis of Ki67 for tissues of xenograft tumors. Amplification: 400 × , scale bar: 20 μm. (H) VEGF, CD147 and MMP-9 protein expressions were detected by Western blot. ** p < 0.01, *** p < 0.001 vs. Colon cancer; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. Low-CN

    Article Snippet: Then the protein samples were separated on the sodium dodecyl sulfate polyacrylamide gel and electroblotted onto polyvinylidene fluoride membranes, which were then blocked with 5% skimmed milk for 2 h. These membranes were immunoblotted using primary antibodies against VEGF (ab32152, 1:1000, Abcam, Cambridge, UK), CD147 (13,287, 1:1000, Cell Signaling Technology, Danvers, MA, USA), MMP9 (ab283575, 1:1000, Abcam), PDE3B (ab99290, 1:1000, Abcam), LIAS (8641, 1:1000, Cell Signaling Technology), DLST (5556, 1:1000, Cell Signaling Technology), FDX1 (ab108257, 1:1000, Abcam), and GAPDH (5174, 1:1000, Cell Signaling Technology) at 4 °C overnight.

    Techniques: In Vivo, Ion Chromatography, Staining, Amplification, Western Blot